CCCCCCCCCCCC CCCCCCCCCCCC CCCC CCCC ooooooo CCCC oo oo nnn CCCC oo oo n n t r i b u t e d Library for the LC ChemStation CCCC oo oo n n CCCC ooooooo CCCC CCCCCCCCCCCC CCCCCCCCCCCC LC ChemStations Revision A.03.xx or higher Date 01/10/94 Macro Contribution #4 Contribution 'Load a chromatographic signal from spectra matrix' Revision History ---------------- 1. A.02.xx original version 2. A.03.xx includes installation update. 3. A.05.xx includes update for data analysis drawing 4. A.06.xx edited to ensure that -ve RTs due to DAD spectra filter do not show in the UI and stop the macro running as expected Contribution Purpose -------------------- To allow the user, interactively through a dialog box, to select the retention time and wavelength range, including the reference wavelength, to extract a chromatographic signal from the spectral data file acquired with the original data. The functionality enables users to extract and process chromatographic signals at wavelengths the were not specified in the original analytical method. Contribution Structure ---------------------- One macro file, 'LoadChro.mac'. Prerequisites ------------- - MS Operating system as supported by the revision of the ChemStation in use - The LC ChemStation software, Rev A.03.xx or higher Installation ------------ * Copy the macro to the ChemStation's executable directory, 'C:\Chem32\CORE' by default. Use --- * Load the macro into the ChemStation using the command... Macro LoadChro.mac, go The command self installs into the File|Load submenu and can be called directly from the pop-up menu. Operation Overview ------------------ The macro tests for the existence of a spectral (*.uv) file associated with the last loaded signal in ChromReg, by trying to load the first spectrum of the file. If successful a dialog box allowing the entry of a RT range and signal and reference wavelength and bandwidth is displayed. The data may be adjusted by the user. They are checked against the boundary conditions specified at the time of data acquisition and, if within the limits, the parameters are passed to the 'LoadChrom' command. Any error conditions are indicated on the ChemStation's message line and the user may correct the data.